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European Development  And Research Academy (EDARA)

    Wednesday, 31 May 2023 14:14

    Isolation and Identification of Proteus Mirabilis Bacteria from Different Pathological Specimen Using Random PCR Method.

    Written by Reem K. Ibrahim, Anmar Nazar Hasan, Bashar Amer Abdulmajeed
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    Isolation and Identification of Proteus Mirabilis Bacteria from Different Pathological Specimen Using Random PCR Method.

    Reem K. Ibrahim, Anmar Nazar Hasan, Bashar Amer Abdulmajeed
    Department of Biology, Collage of Sciences, University of Anbar
    Department of Food Sciences, Collage of Agriculture, University of Anbar
    Ministry of Education, Education Eirectorate in Anbar.

     

    Abstract
    Background polymerase chain reaction (PCR) is a method used to amplify a specific region of DNA, which are repeated many times to produce millions of copies of a specific DNA segment in a random PCR; the primers used in the reaction are not designed to bind specifically to a known target sequence, instead they are designed to bind to any region of the genome. Random PCR is often used to create basic code of random DNA fragments which can be used as starting material for other techniques such as genomic sequencing, cloning or mutation. Methodology Fifty samples of proteus mirabilis were isolated from different sources of pathological conditions such as Urinary tract infection, middle ear infections, and wounds infection, after which they were cultured on their selective media and biochemical test were performed to confirm the purity of the samples.

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